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Isolation of Murine Valve Endothelial Cells
 

Isolation of Murine Valve Endothelial Cells

Article DOI: 10.3791/51860-v • 09:34 min • August 21st, 2014
August 21st, 2014 •
1,2, 2,3
1Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University, 2Center for Cardiovascular and Pulmonary Research, The Heart Center, The Research Institute at Nationwide Children's Hospital, 3Department of Pediatrics, The Ohio State University

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요약
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The ability to isolate heart valve endothelial cells (VECs) is critical for understanding mechanisms of valve development, maintenance, and disease. Here we describe the isolation of VECs from embryonic and adult Tie2-GFP mice using FACS that will allow for studies determining the contribution of VECs in developmental and disease processes.

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Isolation Murine Valve Endothelial Cells Extracellular Matrix Valve Interstitial Cells Valve Endothelial Cells Embryonic Development Valve Integrity Valve Function VEC Disruption Pathological Processes Valve Disease Dysfunction Animal Models Cell-specific Experimentation Small Animal Models Tie2-GFP Reporter Model Green Fluorescent Protein (GFP) Fluorescence Activated Cell Sorting
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