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内皮细胞，细胞连接实时成像过程中中性粒细胞轮回在生理流动
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
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内皮细胞，细胞连接实时成像过程中中性粒细胞轮回在生理流动

19,245 Views

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11:26 min

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August 14, 2014

DOI:

10.3791/51766-v

DOI:
10.3791/51766-v

•

11:26 min
August 14, 2014

•

1 Views
, , ,
1Department of Molecular Cell Biology,Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

Summary

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白细胞交叉使用旁或跨细胞途径的单层内皮细胞​​。我们开发了一个简单的试验，以遵循内源交界的分布VE-cadherin和PECAM-1白细胞跨内皮迁移过程中的生理流动下两路轮回区分。

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Cite this Article

Kroon, J., Daniel, A. E., Hoogenboezem, M., van Buul, J. D. Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow. J. Vis. Exp. (90), e51766, doi:10.3791/51766 (2014).

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