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Chapters
- 00:05Title
- 01:46Preparation of Acute-hippocampal Slices
- 03:00Whole Cell Patch-clamp Recording from Fast-spiking Interneurons
- 05:58Extracellular Electrical Stimulation to Evoke GABAB Receptor-mediated Responses
- 06:56Paired Recordings of Synaptically Coupled Fast-spiking INs and CA1 PCs
- 08:42Visualization and Immunocytochemistry of Fast-spiking Interneurons
- 11:30Results: GABAB Receptor-mediated Currents and Presynapitc Inhibition in Identified Hippocampal Parvalbumin Interneurons
- 13:59Conclusion
Redes corticales son controlados por un conjunto pequeño, pero diverso de interneuronas inhibidoras. Investigación funcional de interneuronas por lo tanto requiere la grabación específica y la identificación rigurosa. Aquí se describe un enfoque combinado que involucra conjunto de células grabaciones de pares individuales o sinápticamente acoplados de neuronas con el etiquetado intracelular, post-hoc morfológica y inmuno análisis.
Tags
Whole-cell Patch-clampHippocampal InterneuronsGABAergic Inhibitory InterneuronsNeuronal CircuitsPhysiological HeterogeneityMorphological HeterogeneityNeurochemical HeterogeneityTransgenic AnimalsFluorescent ProteinsIntrinsic PropertiesSynaptic PropertiesIntracellular Dye LabelingMorphological AnalysisImmunocytochemical Analysis
