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Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
Chapters
- 00:05Title
- 01:38Plating of Human Pluripotent Stem Cells for Differentiation
- 03:08Induction of Differentiation of Neural Crest Cells
- 04:54Replating of Neural Crest Cells for Specification
- 07:37Replating of FACS Sorted Cells
- 08:56Results: Derivation of Neural Crest Cells from hPSCs using a Feeder Free Protocol
- 10:09Conclusion
Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.
