Journal
/
화학
/
La Determinación De Los Planos De Unión Al Hielo De Las Proteínas Anticongelante Por La Afinidad De Plano De Hielo Basado En Fluorescencia
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
Please note that all translations are automatically generated. Click here for the English version.

La Determinación De Los Planos De Unión Al Hielo De Las Proteínas Anticongelante Por La Afinidad De Plano De Hielo Basado En Fluorescencia

8,836 Views

•

08:46 min

•

January 15, 2014

DOI:

10.3791/51185-v

DOI:
10.3791/51185-v

•

08:46 min
January 15, 2014

•

9 Views
, , , , ,
1Department of Biomedical and Molecular Sciences,Queen’s University, 2National Institute of Neurological Disorders and Stroke,Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory,National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition,The Hebrew University of Jerusalem

Summary

Automatically generated

Las proteínas anticongelante (AFP) se unen a planos específicos de hielo para prevenir o retardar el crecimiento del hielo. El análisis de afinidad de plano de hielo basado en fluorescencia (FIPA) es una modificación del método original de grabado en hielo para la determinación de planos de hielo unidos a AFP. Las AFP están etiquetadas fluorescentemente, incorporadas en cristales de hielo macroscópicos individuales y visualizadas bajo luz UV.

Explore More Videos

Antifreeze ProteinsIce-binding PlanesFluorescence-based Ice Plane AffinityIce-etching MethodFluorescent LabelingSingle Ice CrystalIce GrowthAFP Isoforms

Read Article

Cite this Article

Basu, K., Garnham, C. P., Nishimiya, Y., Tsuda, S., Braslavsky, I., Davies, P. Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity. J. Vis. Exp. (83), e51185, doi:10.3791/51185 (2014).

Download .ris file

Copy

Share Video

.

Copy