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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
Chapters
- 00:05Title
- 01:44Preparation of the Bacterial Suspension
- 03:07High-throughput Genome-wide siRNA Screening
- 06:16High-throughput Compound Screening
- 08:27Image Acquisition and Analysis
- 11:10Results: Mtb Colonization in Host Cells is Inhibited by Silencing Dectin-1 and by Isonazid or Rifampicin Treatment
- 14:37Conclusion
Here, we describe a phenotypic assay applicable to the High-throughput/High-content screens of small-interfering synthetic RNA (siRNA), chemical compound, and Mycobacterium tuberculosis mutant libraries. This method relies on the detection of fluorescently labeled Mycobacterium tuberculosis within fluorescently labeled host cell using automated confocal microscopy.
