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Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry
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Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

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09:18 min

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September 05, 2013

DOI:

10.3791/50757-v

DOI:
10.3791/50757-v

•

09:18 min
September 05, 2013

•

2 Views
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1Graduate Program of Environmental Science,Arkansas State University, 2Department of Biological Sciences,Arkansas State University, 3Arkansas Biosciences Institute and College of Agriculture and Technology,Arkansas State University

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Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

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Pannkuk, E. L., Risch, T. S., Savary, B. J. Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry. J. Vis. Exp. (79), e50757, doi:10.3791/50757 (2013).

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