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Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
Chapters
- 00:05Title
- 01:38Embryo Collection and Preparation
- 04:33Embryo Wounding
- 06:18Embryo Microinjection
- 07:34Formaldehyde Fixation Notes
- 09:00Gene Expression at Wound Sites
- 10:45Conclusion
The embryonic epidermis of very late stage Drosophila embryos provides an in vivo system for rapid puncture wound response analysis and can be combined with genetic manipulations or chemical microinjection treatments to advance studies in wound healing for translation into mammalian models.
