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1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department,BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 4Medical Faculty,Lyon 1 University, 5Data and Knowledge Laboratory,BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon
Chapters
- 00:05Title
- 02:42Handling of Prostrate Tissues
- 04:03RNA Extraction, Purification, and Quality Control
- 05:53Target Preparation
- 07:33Hybridization to the HERV Chip Microarray
- 09:24Scanning and Data Analysis
- 10:14Results: Identification of Active HERV Loci in Prostrate Tissues
- 11:49Conclusion
Menneske endogene retrovirus (Herv), som opptar 8% av det menneskelige genom, beholde knappe koding kapasiteter, men hundre tusen lange terminal gjentar (ltr). En tilpasset Affymetrix microarray er designet for å identifisere individuelle Herv locus uttrykk og ble brukt på prostata kreft vev som en proof of concept for fremtidige kliniske studier.
