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Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
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November 02, 2013
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1Joint Unit Hospices de Lyon-bioMérieux, 2Medical Diagnostic Discovery Department,BioMérieux, 3Department of Pathology and Cytology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 4Medical Faculty,Lyon 1 University, 5Data and Knowledge Laboratory,BioMérieux, 6Department of Biochemistry and Molecular Biology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon, 7Department of Urology, Centre Hospitalier Lyon Sud,Hospices Civils de Lyon
Summary
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Human endogenous retroviruses (HERV), which occupy 8% of the human genome, retain scarce coding capacities but a hundred thousand long terminal repeats (LTRs). A custom Affymetrix microarray was designed to identify individual HERV locus expression and was used on prostate cancer tissues as a proof of concept for future clinical studies.
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Pérot, P., Cheynet, V., Decaussin-Petrucci, M., Oriol, G., Mugnier, N., Rodriguez-Lafrasse, C., Ruffion, A., Mallet, F. Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer. J. Vis. Exp. (81), e50713, doi:10.3791/50713 (2013).
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