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Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
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Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
Chapters
- 00:05Title
- 01:48Plating and Maintenance of Human Microvascular Endothelial Cells (HMVEC)
- 02:33Neutrophil Isolation using Polymorphprep
- 04:31Neutrophil Labeling with Calcein AM
- 05:47Neutrophil Binding Assay
- 07:43Results: Measuring Neutrophil Adhesion to Activated Primary HMVEC under Static Conditions
- 10:57Conclusion
Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.
