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Kweken Microglia van de neonatale en volwassen centrale zenuwstelsel
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JoVE 신문 면역학 및 감염병학
Culturing Microglia from the Neonatal and Adult Central Nervous System
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Kweken Microglia van de neonatale en volwassen centrale zenuwstelsel

27,134 Views

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11:28 min

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August 09, 2013

DOI:

10.3791/50647-v

DOI:
10.3791/50647-v

•

11:28 min
August 09, 2013

•

28 Views
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1Program in Neuroscience,Stony Brook University, 2Department of Pharmacological Sciences,Stony Brook University, 3Program in Molecular and Cellular Pharmacology,Stony Brook University

Summary

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Schetsen we methoden voor de efficiënte en snelle isolatie / cultuur van levensvatbare microglia uit de neonatale cerebrale cortex en volwassen ruggenmerg. De dissectie en beplating van corticale microglia kan worden bereikt binnen 90 minuten, met de daaropvolgende microglia oogst plaatsvindt ~ 10 dagen na de eerste dissectie.

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Cite this Article

Bronstein, R., Torres, L., Nissen, J. C., Tsirka, S. E. Culturing Microglia from the Neonatal and Adult Central Nervous System. J. Vis. Exp. (78), e50647, doi:10.3791/50647 (2013).

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