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Immunologie und Infektion
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Culturing Microglia from the Neonatal and Adult Central Nervous System
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Culturing Microglia from the Neonatal and Adult Central Nervous System

Culturing Microglia from the Neonatal and Adult Central Nervous System

27,208 Views

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11:28 min

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August 09, 2013

DOI:

10.3791/50647-v

DOI:
10.3791/50647-v

•

11:28 min
August 09, 2013

•

28 Views
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1Program in Neuroscience,Stony Brook University, 2Department of Pharmacological Sciences,Stony Brook University, 3Program in Molecular and Cellular Pharmacology,Stony Brook University

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We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.

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Cite this Article

Bronstein, R., Torres, L., Nissen, J. C., Tsirka, S. E. Culturing Microglia from the Neonatal and Adult Central Nervous System. J. Vis. Exp. (78), e50647, doi:10.3791/50647 (2013).

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