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培养小胶质细胞的新生和成年的中枢神经系统
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Culturing Microglia from the Neonatal and Adult Central Nervous System
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培养小胶质细胞的新生和成年的中枢神经系统

27,134 Views

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11:28 min

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August 09, 2013

DOI:

10.3791/50647-v

DOI:
10.3791/50647-v

•

11:28 min
August 09, 2013

•

28 Views
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1Program in Neuroscience,Stony Brook University, 2Department of Pharmacological Sciences,Stony Brook University, 3Program in Molecular and Cellular Pharmacology,Stony Brook University

Summary

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我们概述方法从新生儿大脑皮层和脊髓成人的可行的小胶质细胞的高效和快速隔离/文化。解剖和电镀皮质小胶质细胞可以在90分钟内完成，与随后发生〜10天的初始剥离后的小胶质细胞的收获。

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Cite this Article

Bronstein, R., Torres, L., Nissen, J. C., Tsirka, S. E. Culturing Microglia from the Neonatal and Adult Central Nervous System. J. Vis. Exp. (78), e50647, doi:10.3791/50647 (2013).

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