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Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
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Chapters
- 00:05Title
- 01:36Preparation of the Poloxamer 407 Solution
- 02:58Preparation of the 3D Printer
- 04:24Optimization of the Printing Parameters
- 06:42Printing and Elution of the Reverse Mold and Filling of the Voids
- 09:38Results: Patterned Two-component Hydrogel Printing
- 10:21Conclusion
A bioprinter was used to create patterned hydrogels based on a sacrificial mold. The poloxamer mold was backfilled with a second hydrogel and then eluted, leaving voids which were filled with a third hydrogel. This method uses fast elution and good printability of poloxamer to generate complex architectures from biopolymers.
