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Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
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Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

12,162 Views

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11:46 min

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May 16, 2013

DOI:

10.3791/50400-v

DOI:
10.3791/50400-v

•

11:46 min
May 16, 2013

•

2 Views
, ,
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney , 2The MARCS Institute,University of Western Sydney, 3Discipline of Physiology, School of Medical Sciences, Sydney Medical School and Bosch Institute,University of Sydney

Summary

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This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.

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Dynamic ClampSynaptic ConductanceArtificial ConductanceRetinal Ganglion CellsPatch ClampVoltage ClampCurrent ClampNeuronal ExcitabilityExcitatory InputsInhibitory InputsAlpha Functions

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Cite this Article

Huang, J. Y., Stiefel, K. M., Protti, D. A. Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells. J. Vis. Exp. (75), e50400, doi:10.3791/50400 (2013).

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