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Design and Use of Multiplexed Chemostat Arrays
Chapters
- 00:01Title
- 01:12Design and Use of Multiplexed Chemostat Arrays
- 01:18How the Ministats Work
- 02:03Anatomy of the Array
- 03:11How to Clean and Assemble a Ministat Array
- 03:40Marking Culture Tubes at the Desired Working Culture Volume
- 04:11Making the Cork Assembly
- 05:48Setting Up the Gas Washing Bottle to Hydrate Air and Prevent Evaporation
- 06:39Use of a 4-Port Manifold to Divide Air Flow Between Multiple Chambers
- 07:35Setting the Peristaltic Pump and Heating Block
- 08:24Air Tubing Assembly
- 08:50Effluent Tube Assembly
- 09:08Media Tubing Assembly
- 10:21Preparing Effluent Sampling Containers
- 11:01Part Assembly and Autoclaving
- 11:34Connecting all Tubes to the Culture Chamber
- 12:33Preparing the Carboy and Autoclaving
- 12:55Running Experiments
- 15:13Time Zero Sampling
- 16:08Daily Measurements
- 16:52Taking Down an Experiment and Cleaning Up
We developed and validated a small-footprint array of miniature chemostats built from readily available parts for low cost. Physiological and experimental evolution results were similar to larger volume chemostats. The ministat array provides a compact, inexpensive, and accessible platform for traditional chemostat experiments, functional genomics, and chemical screening applications.
