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监测树突状细胞迁移<sup> 19</sup> F /<sup> 1</sup> H磁共振成像
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JoVE 신문 면역학 및 감염병학
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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监测树突状细胞迁移<sup> 19</sup> F /<sup> 1</sup> H磁共振成像

DOI:
10.3791/50251-v

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08:12 min

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March 20, 2013

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, , , , , ,
1Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

Chapters

  • 00:05Title
  • 01:46Labeling and In vivo Application of Dendritic Cells (DC) with 19F (Fluorine)-rich Particles
  • 02:47In vivo 19F/1H (Proton) Magnetic Resonance Imaging (MRI)
  • 05:04Lymph Node Magnetic Resonance Spectroscopy (MRS)
  • 06:20Results: Quantification of 19F-labeled DC Migration In vivo using 19F MRS
  • 07:33Conclusion

Summary

자동 번역

자동 번역

细胞利用磁共振成像跟踪，在过去几年里取得了显着的关注。本协议描述标签的树突状细胞与氟（<sup> 19</sup> F）的丰富的颗粒，这些细胞在体内的应用程序，和监测他们迁移到引流淋巴结的程度<sup> 19</sup> F /<sup> 1</sup> H MRI和<sup> 19</sup> F刘健。

Tags

Magnetic Resonance ImagingMRICell TrackingDendritic CellsFluorine-19PerfluorocarbonCell LabelingCell MigrationIn Vivo ImagingContrast AgentsUSPIOT2 EnhancementBackground-free ImagingCell QuantificationMR Spectroscopy

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