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Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
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Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
Chapters
- 00:05Title
- 01:52Infection of Mice with P. berghei-ANKA
- 06:49Isolation of Brain-sequestered Leukocytes (BSL)
- 09:10Immunophenotyping of BSL by Multicolor Flow Cytometry
- 10:27Representative Analysis of Isolated BSL Populations
- 12:12Conclusion
A method for isolation of adherent inflammatory leukocytes from brain blood vessels of Plasmodium berghei ANKA-infected mice is described. The method allows quantification as well as phenotypic characterization of isolated leukocytes after staining with fluorescent antibodies and subsequent analysis by flow cytometry.
