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Real-time Live Imaging of T-cell Signaling Complex Formation
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JoVE 신문 면역학 및 감염병학
Real-time Live Imaging of T-cell Signaling Complex Formation

Real-time Live Imaging of T-cell Signaling Complex Formation

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10:31 min

June 23, 2013

DOI:

10:31 min
June 23, 2013

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Summary

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We describe a live-cell imaging method that provides insight into protein dynamics during the T-cell activation process. We demonstrate the combined usage of the T-cell spreading assay, confocal microscopy and imaging analysis to yield quantitative results to follow signaling complex formation throughout T-cell activation.

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