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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitative High-throughput Eencellige cytotoxiciteit T cellen
 
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Quantitative High-throughput Eencellige cytotoxiciteit T cellen

Article DOI: 10.3791/50058-v • 09:28 min • February 2nd, 2013
February 2nd, 2013 •
1, 2, 1, 2, 1
1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

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We beschrijven een single-cell high-throughput assay voor cytotoxiciteit van T-cellen te meten wanneer geïncubeerd met tumor doelcellen. Deze werkwijze gebruikt een dichte, elastomeer reeks sub-nanoliter wells (~ 100.000 putjes / array) ruimtelijk beperken de T-cellen en doelcellen met gedefinieerde verhoudingen en is gekoppeld met fluorescentie microscopie effector-target conjugatie en daaropvolgende apoptose controleren.

Tags

Kankerbiologie Immunologie Cellular Biology Moleculaire Biologie Geneeskunde Chemische Technologie cel-en gentechnologie Bioengineering Immunotherapie Adoptieve Microfluidics Nanowell arrays PDMS BioStation T-cellen tumor doelcellen etikettering cytotoxiciteit microscopie analyse
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