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Peptid: MHC-Tetramer-basierte Anreicherung von Epitop-spezifischer T-Zellen
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JoVE 신문 면역학 및 감염병학
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
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Peptid: MHC-Tetramer-basierte Anreicherung von Epitop-spezifischer T-Zellen

17,601 Views

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13:58 min

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October 22, 2012

DOI:

10.3791/4420-v

DOI:
10.3791/4420-v

•

13:58 min
October 22, 2012

•

7 Views
,
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit,Massachusetts General Hospital and Harvard Medical School

Summary

Automatically generated

Dieses Protokoll beschreibt die Verwendung von Peptid: MHC-Tetrameren und magnetische Mikrobeads zur niederfrequenten Populationen von Epitop-spezifischen T-Zellen zu isolieren und zu analysieren durch Durchflusszytometrie. Dieses Verfahren ermöglicht die direkte Untersuchung von endogenen T-Zell-Populationen von Interesse<em> In vivo</em> Experimentellen Systemen.

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Cite this Article

Legoux, F. P., Moon, J. J. Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells. J. Vis. Exp. (68), e4420, doi:10.3791/4420 (2012).

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