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Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

English

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17,615 Views

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13:58 min

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October 22, 2012

DOI:

10.3791/4420-v

DOI:
10.3791/4420-v

•

13:58 min
October 22, 2012

•

7 Views
,
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit,Massachusetts General Hospital and Harvard Medical School

Summary

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This protocol describes the use of peptide:MHC tetramers and magnetic microbeads to isolate low frequency populations of epitope-specific T cells and analyze them by flow cytometry. This method enables the direct study of endogenous T cell populations of interest from in vivo experimental systems.

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Cite this Article

Legoux, F. P., Moon, J. J. Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells. J. Vis. Exp. (68), e4420, doi:10.3791/4420 (2012).

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