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Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
Chapters
- 00:05Title
- 01:24Optical Setup
- 03:18Cell Staining and Recording
- 04:18Online Software Tools to Maximize Spike Detection Efficiency
- 05:44Reconstruction of Spike Timings from Fluorescence Signals
- 07:26Results: Studying the Cortical Function Using Dithered Random-Access Scanning
- 08:12Conclusion
Understanding the function of the vertebrate central nervous system requires recordings from many neurons because cortical function arises on the level of populations of neurons. Here we describe an optical method to record suprathreshold neural activity with single-cell and single-spike resolution, dithered random-access scanning. This method records somatic fluorescence calcium signals from up to 100 neurons with high temporal resolution. A maximum-likelihood algorithm deconvolves the underlying suprathreshold neural activity from the somatic fluorescence calcium signals. This method reliably detects spikes with high detection efficiency and a low rate of false positives and can be used to study neural populations in vitro and in vivo.
Tags
Optical RecordingSuprathreshold Neural ActivitySingle-cell ResolutionSingle-spike ResolutionPopulation Of NeuronsCortical FunctionIntracellular Somatic Calcium ConcentrationElectrical SpikesAction PotentialsTemporal ResolutionAcousto-optical DeflectorsTwo-photon ExcitationFluorescence Calcium RecordingsSpike Detection EfficiencyFalse Positive Spike DetectionTemporal Precision
