In this work we explain the fabrication and use of a microfluidic mixer capable of mixing two solutions in ~8 μs. We also demonstrate the use of these mixers with spectroscopic detection using UV fluorescence and fluorescence resonance energy transfer (FRET).
Waldauer, S. A., Wu, L., Yao, S., Bakajin, O., Lapidus, L. J. Microfluidic Mixers for Studying Protein Folding. J. Vis. Exp. (62), e3976, doi:10.3791/3976 (2012).