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热酚水提取纯化及革兰氏阴性菌脂多糖的可视化
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
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热酚水提取纯化及革兰氏阴性菌脂多糖的可视化

34,039 Views

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05:31 min

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May 28, 2012

DOI:

10.3791/3916-v

DOI:
10.3791/3916-v

•

05:31 min
May 28, 2012

•

25 Views
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1Department of Microbiology, Immunology, & Cancer Biology,University of Virginia Health System

Summary

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我们描述一个净化革兰氏阴性菌脂多糖（LPS）的修改热水溶液中苯酚的提取方法。一旦提取，内毒素可随后通过SDS-PAGE分析，并通过可视化的直接染色或西方免疫。

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Cite this Article

Davis, Jr., M. R., Goldberg, J. B. Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction. J. Vis. Exp. (63), e3916, doi:10.3791/3916 (2012).

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