Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Expression and Purification of the Cystic…
JoVE Journal
Biology

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Biology
Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
 

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

Article DOI: 10.3791/3860-v • 14:57 min • March 10th, 2012
March 10th, 2012 •
1, 1, 1, 1
1Faculty of Life Sciences, University of Manchester

챕터

요약
Automatic Translation

Attempts to express the cystic fibrosis transmembrane conductance regulator (CFTR) in Saccharomyces cerevisiae have, until now, yielded relatively low amounts of protein. This protocol and the associated reagents distributed via the Cystic Fibrosis Foundation should allow the preparation of milligram amounts of this 'difficult' eukaryotic membrane protein.

Tags

Cystic Fibrosis Transmembrane Conductance Regulator CFTR Protein Expression Purification Saccharomyces Cerevisiae Chloride Channel Cystic Fibrosis Protein Structure Protein Activity Recombinant Protein Yeast S. Cerevisiae Proteolysis Problems Cell Growth Conditions Induction Conditions Reagents US Cystic Fibrosis Foundation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter