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면역학 및 감염병학

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单细胞分析枯草芽孢杆菌生物膜，用荧光显微镜和流式细胞仪

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

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单细胞分析枯草芽孢杆菌生物膜，用荧光显微镜和流式细胞仪

DOI:

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13:28 min

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February 15, 2012

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Juan C. Garcia-Betancur, Ana Yepes, Johannes Schneider, Daniel Lopez

¹Institute for Molecular Infection Biology (IMIB),University of Würzburg

Chapters

00:05Title
01:42Labeling B. subtilis and Biofilm Formation Assay
05:30Biofilm Dispersion and Cell Fixation
06:28Fluorescence Microscopy
07:22Quantification of Single Cells Using Flow Cytometry
08:47Results of B. subtilis Biofilm Analysis
13:01Conclusion

Summary

자동 번역

微生物生物膜一般由专门的细胞不同亚群构成。这些亚群的单细胞分析需要使用荧光记者。在这里，我们描述了一个协议，以可视化和监视几个subpopulationswithin<em> B枯草</em>生物膜，用荧光显微镜和流式细胞仪。

Tags

Single-cell Analysis Bacillus Subtilis Biofilms Fluorescence Microscopy Flow Cytometry Extracellular Matrix Proteins Exopolysaccharides Specialized Cells Spatial Organization Temporal Organization Matrix Producers Fluorescent Reporters Surfactin

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