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In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
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March 26, 2012
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In Vitro TranscriptionCappingGaussia Luciferase MRNAHeLa Cell TransfectionRNA PolymeraseDNA TemplatePromoter SequenceTranscription ReactionRibonucleotide TriphosphatesRNase InhibitorMg2+ IonsRNA Structure And Function StudiesSplicingRNAi ExperimentsAntisense RNA AmplificationMicroarray AnalysisRNA Vaccine StudiesRadiolabeled ProbesDye Labeled ProbesT7 High Yield RNA Synthesis KitLinearized Plasmid DNAPCR ProductsSynthetic DNA Oligonucleotides5′ End Cap Structure
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Jani, B., Fuchs, R. In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection. J. Vis. Exp. (61), e3702, doi:10.3791/3702 (2012).
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