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Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall
Chapters
- 00:05Title
- 01:11Larval Dissection
- 02:56Fixation of Larval Fillets
- 03:59Blocking, Staining, and Mounting of Larval Fillets
- 05:56Fluorescent Staining
- 07:22Conclusion
To understand how complex cell shapes, such as neuronal dendrites, are achieved during development, it is important to be able to accurately assay microtubule organization. Here we describe a robust immunohistological labeling method to examine microtubule organization of dendritic arborization neuron sensory dendrites, trachea, muscle, and other Drosophila larva body wall tissues.
Tags
Immunohistological LabelingMicrotubulesSensory Neuron DendritesTracheaeMusclesDrosophila Larva Body WallCell ShapesMicrotubule OrganizationTube MorphogenesisDendritic ArborizationNeuron-class-specific MechanismsDendritic DifferentiationDegenerationCytoskeletal OrganizationImmunological Labeling MethodIn Vivo Microtubule OrganizationFirst Instar LarvaSensory Neuron Dendrite OutgrowthBranching OrganizationMuscles LabelingTrachea Labeling
