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Identifiering av Protein Samverkande Partners Använda Tandem Affinity Purification

JoVE 신문
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JoVE 신문 생물학

Identification of Protein Interacting Partners Using Tandem Affinity Purification

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Identifiering av Protein Samverkande Partners Använda Tandem Affinity Purification

36,956 Views

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10:02 min

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February 25, 2012

DOI:

DOI:

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10:02 min

February 25, 2012

•

4 Views

Dalan Bailey*¹, Luis Urena*¹, Lucy Thorne, Ian Goodfellow

¹Section of Virology, Department of Medicine,Imperial College London

Tags

Protein Interacting Partners Tandem Affinity Purification Identification Of Interacting Proteins Yeast Two Hybrid System Pull Down Assays Immunoprecipitation Mass Spectrometry Identification Double-affinity Tag Mediated Purification TAP Eukaryotic Translation Initiation Factor EIF4E Cellular Translation Factor EIF4E EIF4F Complex Cap-dependent Translation Initiation Protein G Units Streptavidin Binding Peptide Tobacco Etch Virus (TEV) Protease Cleavage Sequence

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