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Identification of Protein Interacting Partners Using Tandem Affinity Purification
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February 25, 2012
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Tandem affinity purification is a robust approach for the identification of protein binding partners. As proof of concept, this methodology was applied to the well-characterized translation initiation factor eIF4E to co-precipitate the host cell factors involved in translation initiation. This method is easily adapted to any cellular or viral protein.
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Bailey, D., Urena, L., Thorne, L., Goodfellow, I. Identification of Protein Interacting Partners Using Tandem Affinity Purification. J. Vis. Exp. (60), e3643, doi:10.3791/3643 (2012).
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