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Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster
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April 26, 2012
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The fruit fly, Drosophila melanogaster, extends its proboscis for feeding, responding to a sugar stimulus from its proboscis or tarsus. I have combined observations of the proboscis extension response (PER) with a calcium imaging technique, allowing us to monitor the activity of neurons in the brain, simultaneously with behavioral observation.
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Simultaneous RecordingCalcium SignalsIdentified NeuronsFeeding BehaviorDrosophila MelanogasterNeuronal ActivityBehavior AnalysisGenetically Encoded Calcium IndicatorsGCaMP1Optogenetic TechniquesThermogenetic TechniquesActivating Identified NeuronsCritical Neurons For Feeding BehaviorProboscis Extension ResponsePERSweet StimulusSensory CellsSuboesophageal GanglionSOGBehavioral ObservationExperimental System
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Yoshihara, M. Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster. J. Vis. Exp. (62), e3625, doi:10.3791/3625 (2012).
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