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A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells
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April 12, 2012
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Cultured muscle cells are an inadequate model to recapitulate innervated muscle in vivo. A functional motor unit can be reproduced in vitro by innervation of differentiated human primary muscle cells using rat embryo spinal cord explants. This article describes how co-cultures of spinal cord explants and muscle cells are established.
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Functional Motor UnitCulture DishCo-cultureSpinal Cord ExplantsMuscle CellsAneural Muscle CellsCell DifferentiationMotor Neuron StimulationIn Vitro StudyNeuromuscular DiseasesInnervationPrimary Muscle CellsFiber ContractionMethod DevelopmentDorsal Root GangliaCross-striated Muscle FibersCulture Medium ExchangeHuman Muscle DevelopmentNeuromuscular Junction
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Arnold, A., Christe, M., Handschin, C. A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells. J. Vis. Exp. (62), e3616, doi:10.3791/3616 (2012).
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