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Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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January 17, 2012
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Parallel-plate Flow ChamberContinuous Flow CircuitEndothelial Progenitor CellsLaminar Flow Shear StressCell AdhesionCell MorphologyHuman Umbilical Cord Blood-derived Endothelial Progenitor CellsPorcine EPCsSmooth Muscle CellsFibroblastsMicrofluidic ChambersSterilizationCell RecoveryDNA ExtractionRNA ExtractionImmunohistochemistryScanning Electron MicroscopyShear Stress AdjustmentPerfusate Flow RateFluid ViscosityChannel Height And Width
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Lane, W. O., Jantzen, A. E., Carlon, T. A., Jamiolkowski, R. M., Grenet, J. E., Ley, M. M., Haseltine, J. M., Galinat, L. J., Lin, F., Allen, J. D., Truskey, G. A., Achneck, H. E. Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress. J. Vis. Exp. (59), e3349, doi:10.3791/3349 (2012).
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