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Epstein - Barr病毒生长转化的淋巴细胞系的建立

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

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Epstein - Barr病毒生长转化的淋巴细胞系的建立

DOI:

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06:38 min

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November 08, 2011

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Joyce Hui-Yuen², Shane McAllister², Siva Koganti, Erik Hill, Sumita Bhaduri-McIntosh^2,3,4

¹Stony Brook Children’s Hospital,State University of New York at Stony Brook, ²Department of Pediatrics,State University of New York at Stony Brook, ³Department of Molecular Genetics,State University of New York at Stony Brook, ⁴Department of Microbiology,State University of New York at Stony Brook

Chapters

01:35Isolation of Peripheral Blood Mononuclear Cells (PBMC)
02:53Infection with Epstein-Barr Virus (EBV)
03:47Expansion and Cryopreservation of Lymphoblastoid Cell Lines (LCL)
04:46Success of Outcome can be Predicted by Flow Cytometric Analysis of Cells
05:58Conclusion

Summary

자동 번역

我们描述了一种用于发电使用EB病毒转化的B细胞株的方法。我们也说明了一种新的分析，可以识别注定要进行改造，早在感染后3天的B细胞。

Tags

Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines EBV Infection B Cell Proliferation Immortalization EBV Latency Virus-driven B Cell Proliferation Tumorigenesis Immunologic Assays Human Monoclonal Antibodies Mitogens Phytohemagglutinin Lipopolysaccharide Pokeweed Mitogen Cyclosporin A T Cell-mediated Killing High Titer EBV Immunosuppressive Agent Peripheral Blood Mononuclear Cells

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