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Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
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Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
Chapters
- 00:05Title
- 01:15Unpacking and Maintenance of EpiAirways
- 02:45Inoculation of EpiAirways
- 03:37NTHi Inoculum Quantification
- 04:16Harvesting of EpiAirways
- 07:05Scanning Electron Microscopy and Bacterial Inoculum Quantification of EpiAirway Co-Cultures
- 07:47Conclusion
This method allows characterization of extended bacterial co-culture with EpiAirways, primary human respiratory epithelial tissue grown at the air-liquid interface, a biologically relevant in vitro model. The approach can be used with any microbe that is amenable to long-term co-culture.
