This method allows characterization of extended bacterial co-culture with EpiAirways, primary human respiratory epithelial tissue grown at the air-liquid interface, a biologically relevant in vitro model. The approach can be used with any microbe that is amenable to long-term co-culture.
Ren, D., Daines, D. A. Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions. J. Vis. Exp. (55), e3261, doi:10.3791/3261 (2011).