Journal
/
생체공학
/
Visualisere Proteiner og makromolekylær komplekser ved Negative Stain EM: fra Grid Forberedelse til Image Acquisition
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

Visualisere Proteiner og makromolekylær komplekser ved Negative Stain EM: fra Grid Forberedelse til Image Acquisition

57,326 Views

•

08:01 min

•

December 22, 2011

DOI:

10.3791/3227-v

DOI:
10.3791/3227-v

•

08:01 min
December 22, 2011

•

7 Views
, ,
1Graduate Group in Biophysics,University of California San Francisco , 2Department of Biochemistry and Biophysics,University of California San Francisco

Tags

VisualizingProteinsMacromolecular ComplexesNegative Stain EMGrid PreparationImage AcquisitionSingle Particle Electron MicroscopyStructural BiologyProtein StructuresCryoEMSample Preparation MethodDried Heavy Metal SaltSpecimen ContrastNegative Stain EMThree-dimensional StructurePurified ProteinsProtein ComplexesHomogeneity/heterogeneityLarge AssembliesQuality EvaluationEM ProtocolCarbon Coated GridsElectron Microscope

Read Article

Cite this Article

Booth, D. S., Avila-Sakar, A., Cheng, Y. Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition. J. Vis. Exp. (58), e3227, doi:10.3791/3227 (2011).

Download .ris file

Copy

Share Video

.

Copy