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Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
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生物工学
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JoVE Journal 生物工学
Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

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08:01 min

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December 22, 2011

DOI:

10.3791/3227-v

DOI:
10.3791/3227-v

•

08:01 min
December 22, 2011

•

7 Views
, ,
1Graduate Group in Biophysics,University of California San Francisco , 2Department of Biochemistry and Biophysics,University of California San Francisco

タグ

VisualizingProteinsMacromolecular ComplexesNegative Stain EMGrid PreparationImage AcquisitionSingle Particle Electron MicroscopyStructural BiologyProtein StructuresCryoEMSample Preparation MethodDried Heavy Metal SaltSpecimen ContrastNegative Stain EMThree-dimensional StructurePurified ProteinsProtein ComplexesHomogeneity/heterogeneityLarge AssembliesQuality EvaluationEM ProtocolCarbon Coated GridsElectron Microscope

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Booth, D. S., Avila-Sakar, A., Cheng, Y. Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition. J. Vis. Exp. (58), e3227, doi:10.3791/3227 (2011).

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