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Het visualiseren van Eiwitten en macromoleculaire complexen door Negative Stain EM: van Grid Voorbereiding op Image Acquisition
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December 22, 2011
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VisualizingProteinsMacromolecular ComplexesNegative Stain EMGrid PreparationImage AcquisitionSingle Particle Electron MicroscopyStructural BiologyProtein StructuresCryoEMSample Preparation MethodDried Heavy Metal SaltSpecimen ContrastNegative Stain EMThree-dimensional StructurePurified ProteinsProtein ComplexesHomogeneity/heterogeneityLarge AssembliesQuality EvaluationEM ProtocolCarbon Coated GridsElectron Microscope
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Booth, D. S., Avila-Sakar, A., Cheng, Y. Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition. J. Vis. Exp. (58), e3227, doi:10.3791/3227 (2011).
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