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Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
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November 11, 2011
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A live fluorescence imaging technique to quantify the replenishment and mobilisation of specific synaptic vesicle (SV) pools in central nerve terminals is described. Two rounds of SV recycling are monitored in the same nerve terminals providing an internal control.
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Quantitative AnalysisSynaptic Vesicle Pool ReplenishmentCultured Cerebellar Granule NeuronsFM DyesNeurotransmitter ReleaseEndocytosisRecycling PoolReadily Releasable PoolReserve PoolAssaySV TrafficClathrin-dependent EndocytosisActivity-dependent Bulk EndocytosisMobilisation MechanismsSV TurnoverCentral Nerve TerminalsHydrophobic Hydrocarbon TailHydrophilic Head GroupFluorescent Probes
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Cheung, G., Cousin, M. A. Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes. J. Vis. Exp. (57), e3143, doi:10.3791/3143 (2011).
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