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Detection of Histone Modifications in Plant Leaves
Chapters
- 00:05Title
- 01:25Crosslinking of Plant Material
- 02:25Isolation of Chromatin
- 04:18Immunoprecipitation
- 05:26Locus-specific DNA Quantitation
- 05:55Benzothiadiazole (BTH) Induced Histone Acetylation on the PR2 Promoter in Arabidopsis
- 06:20Conclusion
A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.
Tags
Histone ModificationsPlant LeavesChromatin StructureGene Expression RegulationChromatin RemodelingDNA MethylationCovalent ModificationsHistones H3 And H4Lysine MethylationArginine MethylationLysine AcetylationSerine PhosphorylationGene ActivationGene RepressionPrimed State Of GeneChromatin Immunoprecipitation (ChIP)Modification-specific AntibodiesReal-time Quantitative PCRC4 Photosynthesis In MaizeSystemic Immunity In Arabidopsis
