/

면역학 및 감염병학

/

体外广角T细胞在小鼠淋巴结切片和共聚焦显微镜成像

JoVE 신문
면역학 및 감염병학

This content is Free Access.

JoVE 신문 면역학 및 감염병학

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

Please note that all translations are automatically generated. Click here for the English version.

体外广角T细胞在小鼠淋巴结切片和共聚焦显微镜成像

16,465 Views

•

09:45 min

•

July 15, 2011

DOI:

DOI:

•

09:45 min

July 15, 2011

•

24 Views

Hélène Salmon^1,2, Ana Rivas-Caicedo^1,2, François Asperti-Boursin^1,2, Camille Lebugle^1,2, Pierre Bourdoncle^1,2, Emmanuel Donnadieu^1,2

¹Institut Cochin,Université Paris Descartes, CNRS (UMR 8104), ²Inserm,U1016, Paris, France

Summary

这个协议描述了一种方法引入到淋巴结切片的荧光图像的T细胞。该技术允许传统的宽视场荧光或共聚焦显微镜的实时分析，T细胞迁移。

Explore More Videos

Ex Vivo Imaging T Cells Lymph Node Slices Widefield Microscope Confocal Microscope Migration Of T Cells Chemokines Two-photon Microscopy Quantitative Information Interactions With Other Cells Slice Assay Fluorescently Labeled T Cells Acutely Prepared Lymph Node Slice Real-time Imaging Natural Environment Mechanisms Underlying T Cell Migration

Read Article