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Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes
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July 15, 2011
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This protocol describes a method to image fluorescent T cells introduced into lymph node slices. The technique permits real-time analyses of T cell migration with traditional widefield fluorescence or confocal microscopes.
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Ex Vivo ImagingT CellsLymph Node SlicesWidefield MicroscopeConfocal MicroscopeMigration Of T CellsChemokinesTwo-photon MicroscopyQuantitative InformationInteractions With Other CellsSlice AssayFluorescently Labeled T CellsAcutely Prepared Lymph Node SliceReal-time ImagingNatural EnvironmentMechanisms Underlying T Cell Migration
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Salmon, H., Rivas-Caicedo, A., Asperti-Boursin, F., Lebugle, C., Bourdoncle, P., Donnadieu, E. Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes. J. Vis. Exp. (53), e3054, doi:10.3791/3054 (2011).
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