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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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September 22, 2011
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High-throughputAutomated PlatformManufacturing Cell LinesProtein TherapeuticsBiopharmaceutical IndustryProduction SystemsDrug SuppliesManual OperationsLabor-intensiveLow-throughputHuman ErrorsBD FACS Aria Cell SorterCloneSelect ImagerTECAN Freedom EVO Liquid Handling SystemCell Line Development ProcessTransfected CellsExpression VectorSelection AgentFluorescence-labeled Anti-human IgG AntibodyFlow Cytometry AnalysisProductive CellsSingle-cell SortingBD FACSAriaColony FormationTime-lapse Digital Images
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Shi, S., Condon, R. G., Deng, L., Saunders, J., Hung, F., Tsao, Y., Liu, Z. A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics. J. Vis. Exp. (55), e3010, doi:10.3791/3010 (2011).
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