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September 22, 2011
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A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.
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High-throughputAutomated PlatformManufacturing Cell LinesProtein TherapeuticsBiopharmaceutical IndustryProduction SystemsDrug SuppliesManual OperationsLabor-intensiveLow-throughputHuman ErrorsBD FACS Aria Cell SorterCloneSelect ImagerTECAN Freedom EVO Liquid Handling SystemCell Line Development ProcessTransfected CellsExpression VectorSelection AgentFluorescence-labeled Anti-human IgG AntibodyFlow Cytometry AnalysisProductive CellsSingle-cell SortingBD FACSAriaColony FormationTime-lapse Digital Images
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Shi, S., Condon, R. G., Deng, L., Saunders, J., Hung, F., Tsao, Y., Liu, Z. A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics. J. Vis. Exp. (55), e3010, doi:10.3791/3010 (2011).
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