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Desarrollo de programas específicos de células de tipo anti-gp120 del VIH aptámeros para la entrega de siRNA
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Chapters
- 00:05Title
- 01:20Preparation of RNA Library
- 03:00In vitro Generation of Aptamers by Selex
- 06:47Monitoring SELEX Progress
- 09:51Isolation and Characterization of New RNA Aptamers Against HIV-1BaL gp120
- 13:27Conclusion
Varios fluoro-2'-aptámeros de ARN del VIH-1BA-L con gp120 nanomol afinidad están aisladas de la biblioteca de ARN por<em> In vitro</em> Procedimiento SELEX. Un nuevo doble función inhibitoria anti-gp120 aptamer-siRNA quimera se crea y se muestra muy prometedora para sistémica anti-VIH.
Tags
Cell-type SpecificAnti-HIVGp120 AptamersSiRNA DeliveryHIV InfectionAntiretroviral AgentsSmall Interfering (si)RNAsTherapeuticsRNAi-basedHIV/AIDSRNA TranscriptsEfficient DeliveryTargeted Intracellular SiRNA Delivery ApproachHIV-1 Gp120 ProteinGlycoprotein EnvelopeViral EntryCD4 CellsAnti-viral StrategyDrug Discovery2′-F Modified Anti-HIV Gp120 RNA AptamersNitrocellulose Filter SELEX Method
