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Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti
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June 17, 2011
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A simple procedure of vibratome sectioning the organ of Corti, followed by immunohistochemistry and confocal microscopy is described. This procedure allows for improved preservation of the fine cytoarchitecture of the mammalian organ of Corti, and consequently allows for accurate quantification of cell types.
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Vibratome SectioningCytoarchitectureMammalian Organ Of CortiCellular MosaicMechanosensory Hair CellsNonsensory Supporting CellsCross-sectioningHistological ProceduresParaffin SectioningCryo-sectioningImmunohistochemical StainingVibratome SectionsConfocal MicroscopyImmunohistochemical AnalysisMutant Organs Of Corti
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Shim, K. Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti. J. Vis. Exp. (52), e2793, doi:10.3791/2793 (2011).
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