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Chapters
- 00:05Title
- 00:43Introduction
- 01:15Neuron Transfection
- 02:44FRAP Experiment on a Spine
- 04:40Data Analysis
- 05:28Representative Results: FRAP Measurements of EGFP Fluorescence in a Spine from a Cultured Hippocampal Neuron
- 06:13Conclusion
FRAP has been used to quantify the mobility of Green Fluorescence Protein (GFP)-tagged proteins in cultured cells. We examined the mobile/immobile fractions of the GFP by analyzing the fluorescence recovery percentage after photobleaching. In this study, FRAP was performed at spines of hippocampal neurons.
Tags
Fluorescence Recovery After Photobleaching (FRAP)GFP-tagged ProteinsDendritic SpinesCultured Hippocampal NeuronsMobilityExcitation LaserFluorescence Recovery RateProtein Turnover RateEGFP VectorZeiss 710 Confocal MicroscopePhotobleachingTime Lapse ImagesMobile FractionImmobile FractionImageJ SoftwareGraphpad Software
